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1When we send semen out to our clients the specimens are cryopreserved in screw-top vials.
2In Experiment 2, cooled-transported semen was centrifuged at room temperature and cryopreserved in three semen freezing extenders.
3Semen was collected from five rams and cryopreserved in a Tris-based extender supplemented with glycerol and egg yolk as the main cryoprotectants.
4After dilution, semen samples were cryopreserved in 0.5 mL straws for 10 days in a liquid nitrogen tank.
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