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This study represents the first use of hiPSC technology to model the effects of spaceflight on humancardiomyocyte structure and function.
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The lack of appropriate humancardiomyocyte-based experimental platform has largely hindered the study of cardiac diseases and the development of therapeutic strategies.
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This system should permit identification of regulatory pathways for humancardiomyocyte proliferation and may facilitate expansion of cardiomyocytes from human ES cells for therapeutic purposes.
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Methods and results: Herein, we demonstrate that mouse and humancardiomyocytes express the TWEAK receptor Fn14.
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The effect of purified KCNQ1 antibodies on humancardiomyocytes derived from induced pluripotent stem cells was then studied.
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Currently, the immaturity of humancardiomyocytes derived from stem cells limits their utility for regenerative medicine and biological research.
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Moreover, whether these Ca(2+) release channels are present and play a critical role in humancardiomyocytes remains to be defined.
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We validated the technique on humancardiomyocytes either freshly isolated or selectively excised from fixed sections of human myocardium by Laser Capture Microdissection.
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We validate optical actuation by virally introducing optogenetic drivers in rat and humancardiomyocytes or through the modular use of dedicated light-sensitive somatic 'spark' cells.
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Humancardiomyocyte progenitor cells (hCMPCs) are cardiac progenitor cells that are unique for their efficient differentiation into beating cardiomyocytes without requiring co-culture with neonatal cardiomyocytes.