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Flux- dependentinactivation that arises from functional coupling between the inner gate and the selectivity filter is widespread in ion channels.
13
Two potential mechanism-based inhibitors were tested with recombinant taxadiene synthase but neither provided time- dependentinactivation nor afforded more than modest competitive inhibition.
14
A new inactivator of the enzyme, 6-chloro-3-hydroxyanthranilic acid, was synthesized and was shown to exhibit time- dependentinactivation.
15
We therefore conclude that the dependence of activity- dependentinactivation on divalent cation influx cannot be attributed to the known form of calcium-dependentinactivation.
16
Their voltage- and Ca(2+)- dependentinactivation kinetics are slower than in other tissues but the underlying molecular mechanisms are not yet understood.