Method in molecular biology; variant of polymerase chain reaction (PCR), is a technique commonly used in molecular biology to detect RNA expression.
1 This might complement future efforts to standardize MDR1 detection methods using RT - PCR .
2 The use and validity of RT - PCR results as gold standard are discussed.
3 Results: The transfection efficiency was confirmed with real-time RT - PCR and Western blotting.
4 Only patients with a confirmatory SARS-CoV-2 RT - PCR were included in the study.
5 Significantly altered microRNAs were validated in independent sample sets via quantitative RT - PCR .
6 RT - PCR and Western blot were performed to examine mRNA and protein levels.
7 Selected genes were verified by quantitative RT - PCR using a larger sample set.
8 MTT and RT - PCR were used for cell proliferation and apoptosis-related proteins analyses.
9 The use of real-time RT - PCR avoided artifacts commonly encountered with microarray technologies.
10 RT - PCR analysis demonstrated the expression of OsLPT1 in both leaves and roots.
11 In the reported experiments, multiplexing did not affect the efficiency of RT - PCR .
12 PKM2 expression in cells was analyzed by Western blotting and real-time RT - PCR .
13 The silencing effects of each siRNA were quantitatively assessed by Semi-Quantitative RT - PCR .
14 Gene expression in uterus and in liver was assessed using realtime RT - PCR .
15 Genes were validated by relative quantitative RT - PCR and RNA in situ hybridization.
16 Based on these annotations, MSDIN genes were found and verified by RT - PCR .
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