Method in molecular biology; variant of polymerase chain reaction (PCR), is a technique commonly used in molecular biology to detect RNA expression.
1This might complement future efforts to standardize MDR1 detection methods using RT-PCR.
2The use and validity of RT-PCR results as gold standard are discussed.
3Results: The transfection efficiency was confirmed with real-time RT-PCR and Western blotting.
4Only patients with a confirmatory SARS-CoV-2 RT-PCR were included in the study.
5Significantly altered microRNAs were validated in independent sample sets via quantitative RT-PCR.
6RT-PCR and Western blot were performed to examine mRNA and protein levels.
7Selected genes were verified by quantitative RT-PCR using a larger sample set.
8MTT and RT-PCR were used for cell proliferation and apoptosis-related proteins analyses.
9The use of real-time RT-PCR avoided artifacts commonly encountered with microarray technologies.
10RT-PCR analysis demonstrated the expression of OsLPT1 in both leaves and roots.
11In the reported experiments, multiplexing did not affect the efficiency of RT-PCR.
12PKM2 expression in cells was analyzed by Western blotting and real-time RT-PCR.
13The silencing effects of each siRNA were quantitatively assessed by Semi-Quantitative RT-PCR.
14Gene expression in uterus and in liver was assessed using realtime RT-PCR.
15Genes were validated by relative quantitative RT-PCR and RNA in situ hybridization.
16Based on these annotations, MSDIN genes were found and verified by RT-PCR.