The expression of two other genes was confirmed by rtPCR.
2
Among them, we measured by q-RTPCR the overexpression of 11 of 14 genes.
3
Both patients had positive RTPCR for SARS-COV-2 in the second round of testing but the first assay tested was negative.
4
In addition, mRNA expression of estrogen sensitive genes in the uteri of these rats was measured using real time rtPCR.
5
The same safety protocols and process will apply with these operating as drive or walk-through testing centres for the rtPCR Covid-19 Antigen testing.
1
Microarray results were confirmed by real-time reversetranscriptionpolymerasechainreaction.
2
Alternative mRNA splicing of fibronectin was studied by reversetranscriptionpolymerasechainreaction.
3
The results were further confirmed at mRNA level by reversetranscriptionpolymerasechainreaction.
4
Expression of the transporter gene MDR1 was performed by quantitative reversetranscriptionpolymerasechainreaction.
5
The fusions were confirmed by specific amplification of the genomic breakpoints using reversetranscriptionpolymerasechainreaction.
1
Three transcripts of PHF14 in human cell lines were identified by reversetranscriptasepolymerasechainreaction.
2
Expression of nutrition-sensitive genes was explored by quantitative reversetranscriptasepolymerasechainreaction in rat prefrontal cortex.
3
We employed a multimarker reversetranscriptasepolymerasechainreaction assay that demonstrates improved sensitivity over a single-marker approach.
4
Independent validation of these findings by quantitative real-time reversetranscriptasepolymerasechainreaction did not confirm these effects.
5
Methods: UT receptor expression as protein and as mRNA were assessed by Western blot and reversetranscriptasepolymerasechainreaction.
1
Methods: Nucleocapsid gene was obtained by reversetranscriptionandpolymerasechainreaction from a SARS patient and inserted into the vector pFastBacHTa expressing baculovirus.
2
Although modal lengths of RNA fragments were shorter, reversetranscriptionandpolymerasechainreaction was able to amplify amplicons in range of 300 bp.
Uso de rt-pcr em inglês
1
This might complement future efforts to standardize MDR1 detection methods using RT-PCR.
2
The use and validity of RT-PCR results as gold standard are discussed.
3
Results: The transfection efficiency was confirmed with real-time RT-PCR and Western blotting.
4
Only patients with a confirmatory SARS-CoV-2 RT-PCR were included in the study.
5
Significantly altered microRNAs were validated in independent sample sets via quantitative RT-PCR.
6
RT-PCR and Western blot were performed to examine mRNA and protein levels.
7
Selected genes were verified by quantitative RT-PCR using a larger sample set.
8
MTT and RT-PCR were used for cell proliferation and apoptosis-related proteins analyses.
9
The use of real-time RT-PCR avoided artifacts commonly encountered with microarray technologies.
10
RT-PCR analysis demonstrated the expression of OsLPT1 in both leaves and roots.
11
In the reported experiments, multiplexing did not affect the efficiency of RT-PCR.
12
PKM2 expression in cells was analyzed by Western blotting and real-time RT-PCR.
13
The silencing effects of each siRNA were quantitatively assessed by Semi-Quantitative RT-PCR.
14
Gene expression in uterus and in liver was assessed using realtime RT-PCR.
15
Genes were validated by relative quantitative RT-PCR and RNA in situ hybridization.
16
Based on these annotations, MSDIN genes were found and verified by RT-PCR.